Pluriselect特约代理商|43-10010-70 p

原创：western protocol

of a 10% gel 4 ml H2O 3.3ml 30% acrylamide/bisacrylamide (29:1 mix) 2.5 ml1.5M Tris(PH 8.8) 10% SDS 100 µl 10% ammonium persulfate 100 µl TEMED 8 µl 2.  Stacking gel: 4 ml 2.7ml water 1 ml 0.67ml 30% acrylamide/bisacrylamide (29:1 mix) 0.ml 1.0M

WESTERN PROTOCOL

For 500,000 cells, lyse with 20 µl. Spin at 14,000 rpm (16,000 g) in an Eppendorf microfuge for 10 min at 4°C. Transfer the supernatant to a new tube and discard the pellet. Determine the protein concentration (Bradford assay, A280

【共享】 蛋白质含量测定法/蛋白质沉淀法/Western免疫印迹

with 100 µl lysis buffer on ice for 10 min. For 500,000 cells, lyse with 20 µl. Spin at 14,000 rpm (16,000 g) in an Eppendorf microfuge for 10 min at 4°C. Transfer the supernatant to a new tube and discard the pellet. Determine the protein