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文献和实验Cell counting with an hemacytometer.
). Carefully and continuously fill evenly at once the hemacytometer chamber. Leave undisturbed for 1-2 min. (If you need to leave for longer, use a humid chamber). This will allow deposition of the cells on the counting plane. Count under the microscope
Cell counting with an hemacytometer.
for 1-2 min. (If you need to leave for longer, use a humid chamber). This will allow deposition of the cells on the counting plane. Count under the microscope two 1x1x0.1 mm areas (delimited by a double line; nine small squares), from two chambers
Chemotaxis Assay Using Microchemotaxis Chambers (Modified Boyden Chamber)
all at once. 8. Place the chamber on a level surface in a 37°C, 5% CO2 incubator. Incubate for 30 minutes to 3 hours (see Hint #7 ). E. Fixing, Staining and Counting of Migrated Cells 1. Hold the assembly together with your hands and remove the screws. Turn
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