vitanavitech特约代理商|VN900RK One-

Two-Step RT-PCR protocol

•Can be more sensitive than one-step RT- PCR Two-step Protocol Step One: Reverse Transcription Note: Wear gloves at all times to avoid RNase contamination Reagents: RETROscript™ kit from Ambion, Catalogue # 1710 -Reverse Transcriptase MMLV-RT (100 units/μl

Protocol for competitive RT-PCR

volume of a 10x PCR standard buffer (15 mM MgCl2; 100 mM Tris /HCl, pH 8.3; 500 mM KCl) are added to a total volume of 50 µl. The PCR is run in the thermal cycler GeneAmp 9600 (Perkin Elmer). The PCR products are then separated on a 1.5% agarose gel

在RT-PCR中避免DNA污染（Avoiding DNA Contamination in R

was isolated according to protocol by five different methods. 0.5 µg RNA was used in RT-PCR reactions with Ambion's RETROscript® Kit. PCR reactions were performed with 5 µg RNA. 10 µl of each reaction was electrophoresed on a 2% agarose gel and stained