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文献和实验Preparation of Mitochondria from Rat Liver
should be cubed, weighed, and divided into two equal portions, each weighing 3 to 5 grams. For homogenization, the ratio of homogenizing medium to tissue is imporant, as is the depth of liquid in the container when using a Polytron type homogenizer
, each weighing 3 to 5 grams. For homogenization, the ratio of homogenizing medium to tissue is imporant, as is the depth of liquid in the container when using a Polytron type homogenizer. We have had great success by mincing the tissue 'dry' in a 50 ml plastic
Preparing Mitochondria from Rat Liver
' for 10 sec. To remove large cell and tissue fragments and cell nuclei (the 'nuclear pellet'), we centrifuge the homogenate at 500 x g for 10 minutes. Before centrifugation, we top off the homogenate with medium to fill each tube. To bring
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