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文献和实验Rapid Retrieval of DNA Target Sequences by Primer Extension Capture
There is a widespread need for methods to enrich DNA samples for sequences of interest prior to high-throughput sequencing and to reduce the costs associated with a shotgun approach. While useful for targeting megabase-sized regions
Retrieval of Full-Length Functional Genes Using Subtractive Hybridization Magnetic Bead Capture
an efficient and relatively simple approach that combines magnetic bead capture with subtractive hybridization for the rapid and direct recovery of full-length target ORFs. When compared with other PCR-based techniques, a higher degree of specificity
Immobilized pH gradient (IPG) as first dimension
Racket-shaped IPG A new sigmoidal immobilized pH gradient (IPG) was used as the first dimension. It offered high resolution and great reproducibility and allowed high protein loads. Based on our specifications, the non-linear pH gradient
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