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文献和实验Digital Gene Expression by Tag Sequencing on the Illumina Genome Analyzer
both the cost and time required to generate gene expression profiles. The ultra?high?throughput sequencing capability of the Illumina platform allows the cost?effective generation of libraries containing an average of 20 million tags, a 200?fold improvement
Specimen Preparation, Imaging, and Analysis Protocols for Knife-edge Scanning Microscopy
then the protocol in 1.10 above is followed. 4) The mouse is deeply anaesthetized using ketamine and xylazine injected intraperitoneally and then perfused transcardially using 50 mL of room temperature phosphate-buffered saline (pH 7.4), followed
v468 Chapter 7 通过免疫组化来测定β-连环蛋白核定位
9.0. To prepare, dissolve 1.2 g Tris and 0.37 g EDTA in 1 L distilledwater and dissolve by stirring. The resulting solution shouldalready be at the required pH 9.0. 3. Steel pan for boiling slides (we use a high-sided asparagus pan). 4. Hot plate. 5. PBS
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