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文献和实验Compound library HCVcc (stock titer ≥3.25 × 104 ; see protocol 3 and 2) FRET lysis buffer (see recipe ), prechilled to 4°
Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos
embryos. In C. elegans: Modern Biological Analysis of an Organism. Methods in Cell Biology (ed. H. Epstein and D. Shakes) Academic Press, San Diego. I. Materials A. Reagents - Anti-digoxigenin Fab fragment, rhodamine labeled (Boehringer Mannheim
Specimen Preparation, Imaging, and Analysis Protocols for Knife-edge Scanning Microscopy
temperature for 4 hours and then dehydrated through a graded series of ethyl alcohols: 50% and 70% in the refrigerator (4 °C), and 85%, 95% (3 changes), and 100% (4 to 5 changes) in room temperature. The brain is left in each solution for 24 hours.
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