Broadpharm代理商-Azido-PEG35 amin

Revised amino-allyl labeling protocol

, all Tris must be removed from the reaction to prevent the monofunctional NHS-ester Cy-dyes from coupling to free amine groups in solution.     3. Add 450 m L water to each reaction.     C. Cleanup     Add 500 m L neutralized, diluted reaction

Protocol for Reverse Transcription and Amino-allyl Coupling of RNA

by addition of 15 mL 0.1 N HCl.    To continue with the amino-allyl dye coupling procedure, all Tris must be removed from the reaction to prevent the monofunctional NHS-ester Cy-dyes from coupling to free amine groups in solution.   3.  Add 450

Uridine 2′‐Carbamates: Facile Tools for Oligonucleotide 2′‐Functionalization

for preparation of uridine 2??carbamate derivatives based on reaction of 3?,5??disilyl?protected uridine with 1,1??carbonyldiimidazole followed by treatment with an aliphatic amine is presented. A phosphoramidite monomer suitable for automated oligonucleotide