merck milipore特约代理商|ES-006-B E

Immunofluorescence and Fluorescent In Situ Hybridization in Mouse Fibroblasts and Embryonic Stem Cells

Culture undifferentiated or differentiating ES cells on gelatin-coated coverslips for at least 24-48 hours; Wash once in freshly prepared 1X PBS; Fix in freshly made, filter-sterile 3% paraformaldehyde/1X PBS for 10 minutes at RT

Technovit® 9100 Methyl Methacrylate

  4°C Stock solution A ad 500 ml 80 g 3 g / 4 g*     4°C Stock solution B ad 50 ml     4 ml

Evaluation of Proliferation of Neural Stem Cells In Vitro and In Vivo

techniques to evaluate cell proliferation based on EdU incorporation: (a) immunocytochemistry and (b) flow cytometry. EdU, a new thymidine analog, which is detected by a reproducible and sensitive method based on click chemistry, does not require DNA