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上海玉博生物科技有限公司
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文献和实验Culture undifferentiated or differentiating ES cells on gelatin-coated coverslips for at least 24-48 hours; Wash once in freshly prepared 1X PBS; Fix in freshly made, filter-sterile 3% paraformaldehyde/1X PBS for 10 minutes at RT
Technovit® 9100 Methyl Methacrylate
4°C Stock solution A ad 500 ml 80 g 3 g / 4 g* 4°C Stock solution B ad 50 ml 4 ml
Evaluation of Proliferation of Neural Stem Cells In Vitro and In Vivo
techniques to evaluate cell proliferation based on EdU incorporation: (a) immunocytochemistry and (b) flow cytometry. EdU, a new thymidine analog, which is detected by a reproducible and sensitive method based on click chemistry, does not require DNA
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