Broadpharm代理商-Thymidine 5'-Mon

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

(note 7).Materials & Reagents ChIP lysis buffer 30ml final volume:       1.5 ml 1M Hepes       1.05ml 4M NaCl       3ml 10% Triton X (Tx-100)       300µl 10% NAD       24.15ml H20 ChIP high salt buffer       30ml final volume:       1.5ml 1M Hepes    

细胞支原体污染的荧光检测方法

： 1.Hanks’ balanced salt solution without NaHCO3 or phenol red (HBSS, GibcoBRL 21250-014：、bisbenzimide (Hoechst No.33258, Sigma B-2883)、thimerosol (Sigma T-5125)、0.1M citric acid、02M disodium phosphate、glycerol、glacial acetic acid、methanol、strerile H2

支原体检测：DNA萤光染色法

判读。 材料 Hanks’ balanced salt solution without NaHCO3 or phenol red (HBSS, GibcoBRL 21250-014﹚、bisbenzimide (Hoechst No.33258, Sigma B-2883)、thimerosol (Sigma T-5125)、0.1M citric acid、02M disodium phosphate、glycerol、glacial acetic acid、methanol