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文献和实验DAPI Counterstaining Protocols
: Phosphate-buffered Saline (PBS) Optional: antifade reagent (ex. ProLong® Gold or SlowFade® Gold reagent) For flow cytometry: PBS Absolute ethanol Staining buffer (100 mM Tris, pH 7.4, 150 mM NaCl, 1 mM CaCl
reagent such as our SlowFade ® Gold antifade reagent (S36936) or ProLong ® Gold antifade reagent (P36930). E. View the sample using a fluorescence microscope with appropriate filters. 2. Counterstaining Cells in Suspension for Flow
免疫荧光操作（共聚焦显微镜）-Immunofluorescence Protocol for Confocal Microscopy
of Molecular Probes Slowfade Light antifade medium. Pipet about 15 m l on slide and coverslip. For chambered coverglass just put two-three drops in each chamber after wash **This is a "light" fix due to the sensitivity of the antigen(s). Cells
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