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文献和实验线粒体荧光探针大全：TMRM，Mitotracker，JC-1
Carbocyanines Most carbocyanine dyes with short (C1 �C6 ) alkyl chains (Section 22.3) stain mitochondria of live cells when used at low concentrations (~0.5 µM or ~0.1 µg/mL); those with pentyl or hexyl substituents also stain the endoplasmic
Intracellular Immunofluorescent Staining for Flow Cytometry
type and the experimental conditions. For example, to stimulate T cells to produce IFN-γ, TNF-α, IL-2, and IL-4, a combination of PMA (a phorbol ester / PKC activator) and Ionomycin (a calcium ionophore) or anti-CD3 antibodies can be used. To induce IL
Basic Methods of Culturing Drosophila
, 9 days at 25o C, 11 days at 22o C, 19 days at 18o C. For most purposes stocks are maintained by live culture, transferring adults to fresh medium every few generations. Stocks kept at room temperature should be transferred to fresh food every 20 to 30
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