Biolegend特约代理商|422401 RBC Lysi

Cell Surface Immunofluorescence Staining Protocol

(BioLegend Cat. #420301) to 1X working concentration with DI water. Warm to room temperature prior to use. Add 2 ml of 1X RBC lysis solution to whole blood/antibody mixture. Incubate at room temperature for 10 minutes. 4) Centrifuge at 350 X g

人单核细胞和中性粒细胞

Purpose Materials10ml 6% dextran + 7ml citrate/citric acidDextran: T500 --> 6g+100ml PBSCitrate solution: 25g Na Citrate + 8g citric acid + 500 ml PBS 43 ml blood 12 ml RT Histopaque 107718 ml cold H2 O2 ml 10x PBSM199 for HUVECs: 1L powder pocket

DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE

EGTA stock, pH 8.0) in 100 ml PBS, pH 7.2-7.4 Heat about 80ml H2 O to 600 C and add paraformaldehyde; add 1-2 drops of 10M NaOH to get paraformaldehyde in solution. Cool to room temperature, add 10 ml 10X PBS, adjust pH with HCl, add MgCl