QIAGEN凯杰特约代理商|158904 RBC Lysis

BAC DNA Isolation from 200 ml Cultures by a Cleared Lysate Method

8.0). After mixing gently, the solution is incubated at room temperature for 5 minutes.  3、To resuspended cells, 16 ml of alkaline lysis solution (0.2 N NaOH and 1% SDS) is added and after very gently swirling until the solution is homogenous, it is incubated

细胞裂解液（Cell Lysis Solution）的配置方法

=8.0，加ddH2O定容至200ml，高压灭菌备用。 4. 细胞裂解液的配制 称15gCTAB（Hexadecyltrimethylammonium bromide）（先用约600ml ddH2O加热溶解）放入烧杯中，加入75ml 1M Tris－HCL（Ph=8.0），58.5g NaCL（先用少量ddH2O溶解），30ml 0.5M EDTA，定容至1000ml，混合均匀备用。  

ULTIMATE FREEZE-THAW LYSIS FOR MAMMALIAN CELLS

to precipitate when cold).B.SOLUTIONS.FT LYSIS BUFFER For 100 ml600 mM KCl20 ml 3 M KCl20 mM Tris-Cl (pH 7.8)2 ml 1 M Tris-Cl (pH 7.8)20% Glycerol 20 ml glycerolAdd fresh each time:0.4 mg/ml Pefabloc 1:100 of 40 mg/ml stock10 μg/ml Leupeptin 1:1000 of 10 mg/ml