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文献和实验The basics of cell culture as applied to human cells are discussed. Biosafety when working with human tissue, which is often pathogenic, is important. The requirements for a tissue culture laboratory are described, particularly the range
Centrifuge the extract at 3200 rpm in a GSA rotor for 45 minutes. Precipitated material will form a grayish pellet. Filter the supernate. Filter through a 0.22µm filter in a tissue culture hood. For the first filter pass, place all of the glass pre
Microbial contamination is a major issue in cell culture, but there are a range of procedures which can be adopted to prevent or eliminate contamination. Contamination may arise from the operator and the laboratory environment
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