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文献和实验Evaluation of the Frequency of Virus-Specific CD8+T Cells by Cytokine Flow Cytometry
developments, however, is the analysis of antigen-specific T cells. The problems encountered when analyzing antigen-specific T cells are manyfold. One of the major problems is the fact that their frequencies tend to be rather low. Recent data
Tracking Antigen-Specific CD8+ T Cells Using MHC Class I Multimers
) populations of CD8+ T cells specific for a given antigen. It provides the detailed steps for multimer labeling, magnetic enrichment, and cytometric analysis. Additionally, it provides informations for multiplexing experiments in order to achieve simultaneous
Detection and Characterization of Virus-Specific CD8+ T Cells Using the Tetramer Approach
Hepatitis B virus (HBV)-specific CD8+ T cells, or cytotoxic T lymphocytes (CTLs), are believed to play an important role in the control of infection and development of liver injury (1 ). Therefore the quantitative and qualitative analyses
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