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文献和实验Proliferation Assays (BrdU and EdU) on Skeletal Tissue Sections
with specific steps of the cell cycle, but the gold standard is to quantify the percentage of DNA-synthesizing cells. The thymidine analog 5-bromo-2′-deoxyuridine (BrdU) has been widely used in the last decades for this purpose, with the inconvenience
-methylcytosine; and to detect the incorporation of DNA base analogues such as 5-bromo-2′-deoxyuridine (BrdU) or 5-ethynyl-2′-deoxyuridine (EdU) to be used for a meiotic time-course or assaying newly synthesized DNA. Although these techniques have been specifically developed
Determination of the molecular weight of DNA-binding proteins using UV-crosslinking and SDS-PAGE
We describe the use of UV-crosslinking in combination with SDS-PAGE to determine the approximate molecular weight of DNA-binding proteins. A 5-bromo-2′-deoxyuridine (5-BrdU)-substituted, radioactively labeled double-stranded oligonucleotide
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