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文献和实验Designing Expression Plasmid Vectors in E. coli
The production of proteins is one of the main applications of genetic engineering in biotechnology. Even though standard cloning procedures are now routine and a large variety of host-vector systems for gene expression are available
Optimized Gene Silencing by Co-expression of Multiple shRNAs in a Single Vector
vectors containing a single shRNA-expressing sequence sometimes induce only poor knockdown. In order to improve the knockdown efficiency using shRNA, the multiple shRNA-expressing sequences were introduced into a single plasmid vector. Compared
Broad-Host-Range Plasmid Vectors for Gene Expression in Bacteria
This chapter provides methods and insights into the use of broad-host-range plasmid vectors useful for expression of genes in a variety of bacteria. The main focus is on IncQ, IncW, IncP, and pBBR1-based plasmids which have all been used
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