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上海玉博生物科技有限公司
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文献和实验Subculture of Suspension Cell Lines
into suspension into a small volume of medium mechanically with the aid of cell scrapers. Materials Media? pre-warmed to 37ºC (refer to the ECACC Cell Line Data Sheet for the correct medium) 70% ethanol in water (Prod. No. R8382) PBS without Ca2+/Mg2+(Prod
Subculture of Suspension Cell Lines
attached cells with PBS without Ca2+ /Mg2+ (Prod. No. D8537 ) using 1-2ml for each 25cm2 of surface area. Retain the washings. Pipette trypsin/EDTA (Prod. No. T4049 ) onto the washed cell monolayer using 1ml per 25cm2 of surface area. Rotate flask
callus formation decreases to 25% with an increasing concentration of 2,4- d up to 10 mg/L cell suspension cultures are established from zygotic embryo-derived pale-yellow calli using half-strength SH medium supplemented
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