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- RBC bioscience
- MCA1603
- 2025年07月14日
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
) Dissect embryos or small pieces of tissue (e.g. the size of a retina from an adult rat works well with this procedure) into PBS containing 2mM MgCl2 (PBS + Mg2+) on ice. PBS(10X) 80 g NaCl 2 g KCl 11.5g Na2HPO4 2 g KH2PO
Coomassie Brilliant Blue R 250 (0.8% in 50% methanol + 10% acetic acid) Destaining solution (13% methanol + 3% acetic acid) Procedure 1. Mix buffer and taxol
Monoclonal Antibody Production Protocol
towards the opened end with another set of forceps. The cells from inside the spleen will ooze out with very little damage. Stop the process when you are left with a nearly empty, transparent skin. Collect the cells by rinsing with IMDM. Transfer cell suspension
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