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上海玉博生物科技有限公司
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文献和实验Native Chromatin Preparation and Illumina/Solexa Library Construction
Antibody and preimmune serum for ChIP CD4+ T cell Isolation Kit II (human) (Miltenyi Biotec) Alternatively, Dynabeads Untouched Human CD4 T Cells Kit (Invitrogen) can be used (see Steps 5-21). DNA End-Repair Kit (Epicentre Biotechnologies
Dynabeads® Co-Immunoprecipitation Kit
wall, then remove the supernatant. Repeat the wash once more. 11. Long SB Wash: Add 0.8 or 1.6 ml of SB and mix by vortexing or pipetting. Incubate on a roller/rotator at RT for 15 minutes. Place the tube on a magnet, allow the beads
miRNA microarrays(McManus Lab)(芯片)
dish. Plunge the slides rapidly into blocking solution and vigorously shake, keeping the tops of the slides under the level of solution. After 30 seconds of plunge-mixing,put a lid on the glass box, and let shake gently on a rotator for 15 minutes
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