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文献和实验secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
Intracellular Cytokine Staining Protocol
anti-cytokine antibodies with excess amounts of unlabeled antibody. Alternatively, recombinant cytokines can be used for blocking.eBioscience antibodies are available as Functional Grade (FG) Purified (sterile, endotoxin-tested, no azide), Purified
Intracellular Immunofluorescent Staining for Flow Cytometry
concentrations for this application is 0.5-0.06µg/106 cells, 100µl staining volume. eBioscience fluorochrome-labeled anti-cytokine antibodies are also available in 20µl/test sizes. If using these reagents, add 20µl of pre-titrated antibody to the appropriate tube
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