thermo特约代理商|15-0038-42 CD3 Mon

secondary antibody review -- data from 99 publications

cytometry   used as a control to detect cell responses targeted antigen   7       Alexa Fluor 488         7       Cy3         8 goat IgG   Alexa Fluor 488   1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey

Intracellular Cytokine Staining Protocol

anti-cytokine antibodies with excess amounts of unlabeled antibody. Alternatively, recombinant cytokines can be used for blocking.eBioscience antibodies are available as Functional Grade (FG) Purified (sterile, endotoxin-tested, no azide), Purified

Intracellular Immunofluorescent Staining for Flow Cytometry

concentrations for this application is 0.5-0.06µg/106 cells, 100µl staining volume. eBioscience fluorochrome-labeled anti-cytokine antibodies are also available in 20µl/test sizes. If using these reagents, add 20µl of pre-titrated antibody to the appropriate tube