AAT Bioquest特约代理商|AAT-17595 Cy

Blue Native Gel Electrophoresis

with DMSO and then PPO/DMSO like an SDS-gel. (It's a little hard to see the MW stds of gels treated this way; I can see the orange ferritin bands and BSA monomer. Hiroki stains and detains his gels before PPO/DMSO to see the markers. I don't stain in order

Denaturing Agarose Gel Electrophoresis of RNA

, such as those from needle biopsies or from laser capture microdissected samples, result in very low yields. In these cases, it may be impossible to spare 200 ng of RNA to assess integrity. Alternative nucleic acid stains, such as SYBR® Gold and SYBR® Green II RNA gel stain

Northern blotting - gel preparation and treatment(Northern杂交-凝胶制备及处理)

. After denaturation add 3ml of 10x nucleic acid dye loading buffer. Mix and load onto the agarose gel. 3)Separate the RNA samples using lx MOPS buffer as the electrophoresis buffer. 4)Following electrophoresis, if appropriate, visualize the RNA within the gel