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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
precipitate which develops at intermediate rates. The color can be distinguished from the purple color derived from X-Phos + NBT. Adding tetrazolium red or blue makes the product look more purple (less pink). Salmon-phos (Biosynth International) results
. A chromogenic substrate, 5-bromo-4-chloro-3-indolyl phosphate (X-Phos), which is very similar to X-gal, can be employed for detection and leads to production of a blue precipitate (Figure 1). In addition, the tetrazolium salt NBT, (Altman, 1972) can be used
LINEAGE ANALYSIS USING RETROVIRAL VECTORS
. Moreover, the Xgal product and the X-P/NBT precipitate produced by PLAP often are too dark to allow simultaneous detection of another colored precipitate produced by immunohistochemical detection of a cellular antigen. However, occasionally, this will work (e.g
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