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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
Purification Kit according to the manufacturer’s instructions. Alternatively, standard plasmid preparation protocols or kits can also be used. Screening for Ligated Partners 53. Screen the purified plasmids (from Step 52
Gene Knockout Techniques(1) The Knockout Process
column pool DNA, and a small amount from these reactions is used to inoculate the internal primer reactions. Each 384-well plate will accommodate 96 reactions for each of four different screening intervals. Assemble a master mix of sufficient
with SYBR® Green dye. The Cells-to-cDNA™ II Kit produces cDNA from cultured mammalian cells in less than 2 hours. No RNA isolation is required. This kit is ideal for those who want to perform reverse transcription reactions on small numbers of cells
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