AQIX特约代理商|RS-1-125ml AQIX RS-I

基于PCR技术的染色质沉淀分析

of H2O:54 g of Tris base27.5 g of boric acid20 ml of 0.5 M EDTA (pH 8.0)The 0.5x working solution is 45 mM Tris-borate/1 mM EDTA.TBE is usually made and stored as a 5x or 10x stock solution. The pH of the concentrated stock buffer should be approx

Use of the Sleeping Beauty Transposon System for Stable Gene Expression in Mouse Embryonic Stem Cells

for 15 min in a humidified, 5% CO2 incubator at 37ºC, and wash twice with 1X HBSS. For a 10-cm dish, use 5 mL of gelatin solution and 2 x 5 mL of HBSS. Use the 10-cm dishes as soon as they are prepared; T25 flasks can be stored at 4ºC

Using MicroRNAs to Enhance the Generation of Induced Pluripotent Stem Cells

2 flasks 50‐ml conical tubes Irradiator (RS2000, Rad Source)