Candor Bioscience特约代理商|110 125

原创：western protocol

. 4.  When finished, immerse membrane in Blocking buffer and block overnight. H. Antibodies and detection 1.  Incubate with primary antibody diluted in Blocking buffer for 2 hours at room temp. 2.  Wash 3 x 10 min with PBST. 1 x10min secondary washing

抗体稳定剂解决你的抗体保存问题

，5L，10L，50L，100L的超值大包装。 德国Candor Bioscience专业生产免疫优化试剂，如阻断剂替代品LowCross-Buffer，辣根过氧化物酶保护液LowCross-HRP和HRP Protector，抗体（蛋白）稳定剂Antibody Stabilizer，封闭液The Blocking Solution，液体板稳定剂Liquid Plate Sealer，脱离缓冲液Stripping Buffer。这些产品销往世界各地的诊断试剂公司，包括罗氏诊断。

Kamps's Western Blotting Protocol

activity).We use 12-14 microCi (approx.10 microliters)125I-protein A in a volume of 30-35 ml blocking buffer.We reuse this solution 3 times without noticing a reduction in signal (see #6 in Comments). 9)Wash again as in step 7. Damp dry on Whatman 3MM