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上海玉博生物科技有限公司
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文献和实验RNA Purification Protocol for 10-20mg of Animal Tissue
. The precipitated protein and DNA will form a tight pellet. 6. Transfer the supernatant to a new sterile 1.5 mL centrifuge tube that containing 600ul of 100% isopropanol. If the RNA yield is expected to be low, add total 1ug Linear Polyacrylamide (Cat# PR033
Creation and Use of Infectious Virus Vector
Creation and use of your infectious vector: Plate 5 x 105 293T cells in 6 cm2 dishes containing 5 ml of media. (This can be scaled up if desired). The following day set up (use polypropylene tubes
(upstream part = vector) + Part B (downstream part = insert): Part A + vector Forward primer: 5''-TACTAGTAGCGGCCGCTGCAGGCTTC-3'' (specific to the pSB1A2 suffix + 5 bases downstream of the suffix) Part A + vector Reverse primer: last 20 bases
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