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文献和实验Creation and Use of Infectious Virus Vector
Creation and use of your infectious vector: Plate 5 x 105 293T cells in 6 cm2 dishes containing 5 ml of media. (This can be scaled up if desired). The following day set up (use polypropylene tubes
(upstream part = vector) + Part B (downstream part = insert): Part A + vector Forward primer: 5''-TACTAGTAGCGGCCGCTGCAGGCTTC-3'' (specific to the pSB1A2 suffix + 5 bases downstream of the suffix) Part A + vector Reverse primer: last 20 bases
Distilled water Plasmid DNA isolated from bacteria (LAB 1), such as pGFP(R) (Clontech) and pBC(R) (Stratagene) Supplies: Microcentrifuge with adaptors for 600-ul tubes Micropipetter and tips PCR machine and the 600-ul tubes
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