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文献和实验Immunohistochemistry using Anti-Ganglioside Antibodies
supernatant containing antiganglioside mAb overnight at 4°C. The time of incubation may range from 30 min. to overnight as determined by experience. 5. Wash with 1% BSA in PBS 4 times. 6. Incubate in FITC-conjugated goat anti-mouse IgM F(ab
Dual- and Triple-Color Fluorospot
for the other cytokine (Fig. 1). As a second step in the detection, streptavidin conjugated to a red fluorophore (Cy3) and anti-FITC antibodies labeled with a green fluorophore, respectively, are used (10, 11). The resulting spots are subsequently analyzed using
Flow Cytometry: Immunofluorescence Staining of Activated and Resting Human Platelets
for 30 minutes at room temperature.6. Wash two times in PBS and resuspend cell pellet in PBS. Cells may be stored at 4℃ for up to 8 hours.7. Stain activated platelets by direct immunofluorescence using CD62P-FITC, catalog number 555523 (31794×), at 20 μL
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