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Preparation of probes for in situ hybridization

compound could be used. In practice, CY3 (1 vial of monoreactive labelling kit per reaction, Amersham), works very well for as a red fluorophore, and the succinimidyl ester of Oregon green 488 (1mg of compound per reaction, Molecular Probes) as a green dye

Labeling DNA and Preparing Probes

Jilani, A., Ramotar, D., Slack, C., Ong, C., Yang, X.M., Scherer, S.W., and Lasko, D.D. 1999. Molecular cloning of the human gene, PNKP, encoding a polynucleotide kinase 3′‐phosphatase and evidence for its role in repair of DNA strand

In Situ Hybridization to Dissected Gonads with Digoxygenin-Labeled Probes

% hybridization buffer (HB) and incubate 5 min at 48°C (in water bath). 13) Pre-hybridize in 400 ul HB for 1 hr at 48°. 14) Single-stranded DNA probes are made by asymmetric PCR using a single primer (see Patel and Goodman protocol distributed by G