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文献和实验The activation of CD4+ T-cell subsets, Th1 and Th2, is one of the most important focuses of immunology. Th1 and Th2 are differentiated from naive T cells after antigenic stimulation by the influence of cytokines [interleukin 12 (IL
Osteoclast Formation in Mouse Co-cultures
The murine co-culture assay is used to generate mature osteoclasts from bone marrow precursors by culturing them with osteoblasts that are stimulated with 1,25-dihydroxy vitamin D3 and prostaglandin E2 . This assay is used particularly
Application of Spinal Microdialysis in Freely Moving Rats
we demonstrate its contribution on spinal cord neurotransmission knowledge with practical examples studying pain. In particular, (1) we assessed basal (glu) and prostaglandin E2 (PGE2 ) levels in dorsal horn and in cerebrospinal fluid
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