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文献和实验Phosphate Assay Contributor: Jiandi Zhang Date: July 4, 1996 1. Make standards using sodium phosphate at the following uM concentrations: 0, 2, 5, 7, 10, 20, 40, 60, and 80. Use the screw top glass tubes. 2. Dry
Phosphate Assay by Suprya Jaydev
Phosphate Assay by Suprya Jaydev Contributor: Suprya Jayadev Date: Sep. 8, 1994 Reagents Ashing buffer: 10 g Mg(NO3)2 100 ml EtOH 1.5 N HCl stock: 119.7 ml concentrated HCl (11.6M @ 36% by weight
bodies inEscherichia coli . The current work describes a colorimetric assay for measuring RFAP synthase activity, and two modified procedures for expressing recombinant RFAP synthase genes to produce soluble, active enzyme. By lowering the incubation
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