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A High‐Throughput Screening Method for Identification of Inhibitors of the Deubiquitinating Enzyme USP14

function, inhibitors of USP14 can stimulate proteasomal degradation. We recently established a high?throughput screening (HTS) method to identify small?molecule inhibitors specific for USP14. The protocols in this article cover the necessary procedures

Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit

Aspirate the supernatant. Resuspend the cell pellet in MEF medium to a density of 2.5 × 106 cells/mL.          c. Aspirate the gelatin solution from the gelatin coated culture vessel.          d. Add the appropriate amount of MEF medium

Methods for the Detection of D-Amino-Acid Oxidase

al. (5 ) using bovine serum albumin as a standard. It was also determined using a Protein Assay Kit (Bio-Rad, Hercules, CA). D-Amino-acid oxidase activity is finally expressed as the amount of D-alanine oxidized per min per milligram of protein. Hog