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文献和实验Establishment of a Primary Culture
light source; the embryo can be seen as a shadow. Circle the embryo with a pencil. Place the egg in a beaker with the blunt end up, and wash the top with a mild detergent, followed by swabbing with ethanol.
Establishment of a Primary Culture
. This is easily accomplished by holding the egg in front of a bright light source; the embryo can be seen as a shadow. Circle the embryo with a pencil. Place the egg in a beaker with the blunt end up, and wash the top with a mild detergent, followed by swabbing
cells from the stock culture and transfer these aseptically to one of the waiting test tubes of broth. This tube now contains a dilution of 1/100 or 10. Gently but thoroughtly mix the contents and label this tube at 10. 4. Repeat this process
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