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文献和实验ß - GAL Filter Assay streak yeasts to single colony replica plate onto NC-filter (leave 10 sec.) use tweezers to lift filter and slowly submerge in liquid Nitrogen for 1 min place
X-gal staining of embryos Protocol supplied by S. P. Yee (spyee@julian.uwo.ca) and Joe Chan (cchan@hgmp.mrc.ac.uk). Procedure Dissect embryos in PBS Immediately transfer the embryos to fixative at 4oC
Beta-gal Assay Adapted from Current Protocols in Molecular Biology 1. Grow 5ml YEPD cultures to mid-log phase. 2. Centrifuge and resuspend cells in 5ml Z-buffer, then place on ice. 3. Measure OD600. 4. Use straight
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