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文献和实验In situ hybridisation to alpha satellite sequences (chromosome specific)
anti-mouse HRP antibody diluted 1:80 for 40 minutes at 37C We use DAKO P0260 (12) Wash with blocking solution for 10 minutes (13) Incubate with swine anti-rabbit HRP antibody diluted 1:100 for 40 minutes at 37C We use DAKO P0217 (14
ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
from different species and for different isotypes within a species. Tables I may be used to determine relative avidity for polyclonal antibodies of a given species. Table II shows relative affinities for varius isotypes.
for cell lysis: 10 mM Tris-HCl, pH 7.4,50 mM NaCl, 5 mM EDTA, 50 mM NaF, 30 mM Na 4 P 2 O 7 ,and 1% (v/v) Triton-X100. 5. Protease inhibitors and phosphatase inhibitor are added fresheach time with the following final concentrations: 10 μg/mLaprotinin
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