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文献和实验Polyclonal Antibody Production
For injection of two rabbits, your antigen should be in 1 mL of a buffer such as PBS or Tris that contains no chemicals harmful to the rabbit. 100 µg/rabbit of antigen is best, but you can use less and get good results.
Polyclonal Antibody Production
Polyclonal Antibody Production Very useful for rapid and simple generation of antibodies for western blots, ELISA assays, and immunoprecipitation. Rabbit Immunization Initial Preparation Your antigen
Measurement of Aldosterone in Blood
raised in rabbits to aldosterone conjugated at position C3, and iodinated aldosterone. Details are given for optimizing conditions for the second antibody, raised to rabbit immunoglobulins, and carrier protein used in the separation of the antibody bound
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