invitrogen特约代理商|15240096 Antib

Method: Removal of Yeast Contamination from Lymphoblast Cultures

only antibiotic and not the antimycotic.   Solutions:   Wash media: (1 liter) To 1 liter of sterile RPMI 1640 with 2mM L-glutamine, add: 10.0 ml 2.5 M (100X) Hepes buffer 1.2 ml 50 mg/ml gentamicin reagent Filter sterilize

Cell Culture Media and Solutions

    165.0 ml fetal bovine serum, heat inactivated 12.0 ml 200mM (100X) L-glutamine 12.0 ml antimycotic/antibiotic (100X), liquid, Gibco, Cat. No. 600-5240AG Filter sterilize through a 0.22 � cellulose acetate filter and store

细胞培养中的常见污染

change in pH, cloudiness in the medium, sometimes with a slight whiteish film on the cell surface of plates, dishes, or on the bottom of bottles of medium that typically dissipates when the vessel is moved. Under low power(100x)spaces between cells