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文献和实验96-well RNA In Situ Hybridization Protocol
on whole-mount Drosophila embryos at the same time. Jasprien Noordermeer and Casey Kopczynski I.PREPARATION OF EMBRYOS 1. Embryos were collected for 24 hours and washed 3X with 0.02% Triton X (Tx) (15ml for up to 2ml
(or 2-3h @ RT). 8. Transform 100ml competent cells with 10ml ligation reaction; freeze remainder. EB 66mM Tris-HCl, pH 8 0.66mM MgCl2 10X Klenow Mix 20mM Tris-HCl, pH 8100mM MgCl2 S1B 40mM KOAc 300mM NaCl 1.3mM ZnS04 7% Glycerol Ligation Mix 50mM Tris
96-well RNA In Situ Hybridization Protocol
Noordermeer and Casey KopczynskiI.PREPARATION OF EMBRYOS1. Embryos were collected for 24 hours and washed 3X with 0.02% Triton X (Tx)(15ml for up to 2ml embryos in a 50ml falcon tube).2. Dechorionate 5 min. in 50% bleach.3. Wash 3X with 0.02% Tx.4. Fill tube
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