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- 文献和实验
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- 供应商:
上海玉博生物科技有限公司
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文献和实验Method: Removal of Yeast Contamination from Lymphoblast Cultures
media: To 1 liter of sterile RPMI 1640 with 2mM L-glutamine, add: 165.0 ml fetal bovine serum, heat inactivated 12.0 ml 200mM (100X) L-glutamine 12.0 ml antimycotic/antibiotic (100X), liquid, Gibco, Cat. No. 600-5240AG Filter sterilize
Cell Culture Media and Solutions
165.0 ml fetal bovine serum, heat inactivated 12.0 ml 200mM (100X) L-glutamine 12.0 ml antimycotic/antibiotic (100X), liquid, Gibco, Cat. No. 600-5240AG Filter sterilize through a 0.22 � cellulose acetate filter and store
change in pH, cloudiness in the medium, sometimes with a slight whiteish film on the cell surface of plates, dishes, or on the bottom of bottles of medium that typically dissipates when the vessel is moved. Under low power(100x)spaces between cells
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