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- DNA Polymerase technology
- 332
- 2025年07月14日
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上海玉博生物科技有限公司
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文献和实验Northern Blotting方法(Howell Lab)
inverted because the RNA pellets on the bottom. (DNA can be recovered from the CsCl layer.) Cut off the tube bottoms (keep inverted) and resuspend the pellet in 100 µl 0.3 M NaOAc (pH 6) buffer. Transfer to a screw-cap 1.5 ml Eppi. Wash tube
clear, add 175 ml of TE + 0.1% SDS to each tube, and suspend the RNA by pipetting up and down. This should take about 1 minute. Transfer the RNA to a fresh RNase free screw cap eppendorf tube. Wash out the bottom of the ultracentrifuge tube
Wholemount in situ hybridisation
! 9 Refix with fresh 0.2% glutaraldehyde/4% PFA in PBTX for 20 minutes. 10 Wash twice with PBTX for 10 minutes each. 11 Place in a 2ml screw cap eppendorf tube and fill with prehybridisation mix, and allow the embryos to sink
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