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Use of an immobilized monoclonal antibody to examine integrin 51 signaling independent of cell spreading

spreading, we developed a method engaging integrin signaling by use of an immobilized anti-integrin monoclonal antibody (mab) directed against the fibronectin (FN) receptor integrin α5β1. ECV 304 cells were plated onto FN or immobilized mab JBS5 (anti

Generation of Short-Term Murine Natural Killer Cell Clones to Analyze Ly49 Gene Expression

Natural killer (NK) cellsexpress receptors specific for class I major histocompatibility complex (MHC) molecules. In the mouse, the class I specific receptors identified to date belong to the polymorphic Ly49 receptor family. Engagement of Ly49

Hybrid Resistance by Mouse NK Cells In Vitro

of monoclonal antibodies (MAbs) or F(ab′)2 reagents to block those signals. There is already good evidence for negative signals between Ly49 NK cell receptors and their specific class I antigens, e.g., Ly49A and H2-Dd (2 ). Thus, the inability of H2b Ly49