invitrogen特约代理商|A11001 488 Goa

Purification of mAb (IgG)

  Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers

Pathology and Autopsy of a mouse

  Always fill-in a form for each mouse you find/submit and make as many notes as possible about his behaviour, health status or gross features. Always weigh the mouse. if mouse is found dead : Almost invariably, a mouse

Phenotype-specific immunodetection of cyclins using 488/630 nm dual laser flow cytometry

at 4°C. Wash twice with cold staining buffer and add the Cy5-conjugated anti-mouse IgG (available from Jackson ImmunoResearch and Caltag) in a volume of 200 µl.  Incubate for 4 hours at 4°C. Wash once with cold staining buffer and once  with cold