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上海玉博生物科技有限公司
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文献和实验, and then diluted 4- fold with water. For each ligation reaction, two to threePCRs are run in order to generate > 40 µg of PCR products. Each PCR contains 10 µL of the diluted ligation reactions (25 ng of starting DNA) in 100 µL volumes containing 1.0 µM of primer
with water. For each ligation reaction, two to three PCRs are run in order to generate > 40 µg of PCR products. Each PCR contains 10 µL of the diluted ligation reactions (25 ng of starting DNA) in 100 µL volumes containing 1.0 µM of primer (5’-ATT ATG
of Huh7 cell maintenance medium. Using a multichannel pipettor, three 1:10 dilutions can be made by transferring 20 µl between wells down each column, beginning with the undiluted virus row and moving on to the 1:10 row, the 1:100 row, and the 1:1000 row
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