rna medical 代理商|T310 pre-treat

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

and then centrifuge at 14,000 RPM for 3 minutes. Save 20µl of the elutes as (Flag-Input). Next, take the remaining ~80µl of elute to clean tubes containing 40µl of EZVIEW™ Red anti-Flag M2 affinity gel beads (Sigma™) which have been pre-treated with a wash in 250µl

Convenient and Efficient Approach to the Permanent or Reversible Conjugation of RNA and DNA Sequences with Functional Groups

when treated with TBAF. The versatility and uniqueness of 2??O ?(aminooxymethyl)ribonucleosides is demonstrated by the single or double incorporation of a reversible pyrenylated uridine 2??conjugate into an RNA sequence. Furthermore, the conjugation of 2??O

Direct tissue explant method for derivation and study of extravillous trophoblas

with 100 mg/ml collagenase (Sigma type 1A) at 37°C. Collagenase should be reconstituted in DEPC-treated sterile PBS if the isolated cytotrophoblasts are to be used for RNA extraction. Cytotrophoblast cells are released from the collagen by a 10min