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文献和实验A Comprehensive Guide to Sleeping Beauty–Based Somatic Transposon Mutagenesis in the Mouse
PBST (1× PBS with 0.1% Tween 20) Anti‐SB11 antibody (mouse monoclonal clone 324622) (R&D Systems) VectaStain Elite ABC
Transplantation Models to Characterize the Mechanisms of Stem Cell–Induced Islet Regeneration
PE‐labeled anti‐human HLA‐A,B,C antibody (4 µl/200 µl cells; BD Biosciences, cat. no. 555553) Mouse IgG1 FITC antibody (4 µl/200 µl cells; BD Biosciences, cat. no. 555748
Dual- and Triple-Color Fluorospot
stimulatory agents of choice and anti-CD28 (0.1 μg/ml; seeNote 4), in a final volume of 100–150 μl/well. The number of cells per well is dependent on the stimulating agent used (seeNote 5). b. Incubate the plate overnight at 37°C in a humidified
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