ROCKLAND特约代理商|MB-070-010 Block
ing Buffer for Fluorescent Western Blotting 10-PACK (10 x 500 ml) - MB-070-010- ￥3000
- Merck Millipore
- 17-371RF
- 2025年07月15日
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文献和实验SDS-PAGE Western Blotting Protocols
-PAGE, although it does reduce the effective size of the resolving gel. 8. Add upper layer of water-saturated n-butanol to the top of the gel. 9. Let polymerize (this takes ~10-15’). 10. Invert gel to remove n-butanol. Touch with filter paper to wick off residual
milk overnight at 4°C. Generally, phospho-antibodies require blocking reagents diluted in TBS rather than PBS. If previous Western blots had high backgrounds, try a different blocking buffer. Oter blocking reagents which can be used include a) 3�
Western Blotting and Immunostaining
Wash XCell box, blotting module and trays with soap and water before proceeding. Reagents: Transfer buffer 25 mM Tris HCl, 190 mM Glycine 10% Methanol Blocking
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