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文献和实验Western Blotting and Immunostaining
Wash XCell box, blotting module and trays with soap and water before proceeding. Reagents: Transfer buffer 25 mM Tris HCl, 190 mM Glycine 10% Methanol Blocking
milk overnight at 4°C. Generally, phospho-antibodies require blocking reagents diluted in TBS rather than PBS. If previous Western blots had high backgrounds, try a different blocking buffer. Oter blocking reagents which can be used include a) 3�
Western Blotting and Immunostaining Protocol
Wash XCell box, blotting module and trays with soap and water before proceeding. Reagents: Transfer buffer 25 mM Tris HCl, 190 mM Glycine 10% Methanol Blocking buffer 5% non-fat dry milk in TBS-TWEEN 20
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