pierce特约代理商|37537 SuperBlock™

Western Blotting and Immunostaining

Wash XCell box, blotting module and trays with soap and water before proceeding. Reagents: Transfer buffer 25 mM Tris HCl, 190 mM Glycine 10% Methanol Blocking

Western Blotting Protocols

milk overnight at 4°C. Generally, phospho-antibodies require blocking reagents diluted in TBS rather than PBS. If previous Western blots had high backgrounds, try a different blocking buffer. Oter blocking reagents which can be used include a) 3�

Western Blotting and Immunostaining Protocol

Wash XCell box, blotting module and trays with soap and water before proceeding. Reagents: Transfer buffer 25 mM Tris HCl, 190 mM Glycine 10% Methanol Blocking buffer 5% non-fat dry milk in TBS-TWEEN 20