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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
上海玉博生物科技有限公司
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文献和实验Protocol for anti-HA antibody Western Blotting
of interest. 2) Transfer proteins in acrylamide gel to nylon membrane. 3) Block overnight @4℃ in PBS (or TBS, it doesn't matter) with 5% non-fat dry milk. I dilute 2.5 mg in 50 ml. 4) Wash off excess milk sol'n with PBS x3 .(important if you are re-using your antibody
Protocol for anti-HA antibody Western Blotting
room. Expose film for ~2 mins. Notes on the primary antibody: Source - Covance/Babco Description: Crude ascites fluid monoclonal anti-HA antibody (CATLN#135032002, 11mms-101R, 0.5 ml) Handling: Aliquoted into 10ul portions in ―20c
Microarray Approaches for Analysis of Cell Cycle Regulatory Genes
pRB SBF (Swi4, Swi6) E2F-1, E2F-2 MBF (Mbp1, Swi6)
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